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| 基于网络药理学、分子对接及实验验证探讨清咽利喉方治疗急性咽炎的作用机制 |
| Based on Network Pharmacology, Molecular Docking, and Experimental Validation to Explore the Mechanism of Qingyan Lihou Formula in Treating Acute PharyngitisCAI Tingting1,2, WANG Nannan1,2, LIU Xia1,2, ZHU Wanping1,2# |
| 投稿时间：2026-03-25 修订日期：2026-03-25 |
| 中文关键词: 网络药理学 清咽利喉方 急性咽炎 分子对接 |
| 英文关键词: Network pharmacology Qingyan Lihou formula Acute pharyngitis Molecular docking |
| 基金项目:国家中医药管理局科技司-浙江省中医药管理局共建科技计划重大项目(GZY-ZJ-KJ-23005)；浙江省中医药科技计划项目(2024ZR058) |
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| 中文摘要: |
| 目的:基于网络药理学与分子对接技术探讨清咽利喉方治疗急性咽炎的作用机制,并通过动物实验进行验证。方法:利用TCMSP、Herb等数据库筛选清咽利喉方的活性成分及作用靶点,结合GeneCards、Drugbank数据库获取急性咽炎相关靶点,取交集后构建“中药-活性成分-靶点”网络及蛋白质相互作用(PPI)网络,进行GO功能及KEGG通路富集分析,并通过分子对接预测关键成分与核心靶点的结合能力。动物实验采用15%氨水喷雾法建立大鼠急性咽炎模型,随机分为正常对照组、模型对照组、蒲地蓝口服液组及清咽利喉方高、低剂量组,给药6天后观察咽部表观状态及组织病理学变化,采用Wes技术检测咽部组织中PI3K、p-PI3K、AKT及p-AKT蛋白表达水平。结果:网络药理学筛选出清咽利喉方活性成分62个,对应急性咽炎相关靶点208个,核心靶点包括AKT1、PI3K、IL6等,KEGG富集分析显示PI3K-AKT信号通路为关键通路。分子对接结果表明,槲皮素、甲基麦冬黄酮A等活性成分与PI3K、AKT靶点蛋白结合能均小于-6.0 kcal/mol,亲和力良好。动物实验表明,与模型对照组比较,清咽利喉方各剂量组大鼠咽部分泌物、充血及肿胀状态明显改善,咽部组织病理损伤减轻；咽部组织中PI3K、p-PI3K、AKT及p-AKT蛋白表达水平显著降低(P<0.05,0.01)。结论:清咽利喉方可能通过槲皮素、麦冬黄酮类等活性成分,调控PI3K-AKT信号通路,抑制炎症反应,发挥治疗急性咽炎的作用。 |
| 英文摘要: |
| Objective: To explore the mechanism of Qingyan Lihou Formula in treating acute pharyngitis based on network pharmacology and molecular docking, and to verify the findings through animal experiments. Methods: Active ingredients and corresponding targets of Qingyan Lihou Formula were screened using databases such as TCMSP and Herb. Targets for acute pharyngitis were obtained from the GeneCards and Drugbank databases. After intersecting the targets, a “traditional Chinese medicine - active ingredient - target” network and a protein - protein interaction (PPI) network were constructed. GO functional annotation and KEGG pathway enrichment analyses were performed, and molecular docking was used to predict the binding affinity between key components and core targets. For the animal experiment, a rat model of acute pharyngitis was established by 15% ammonia spray. Rats were randomly divided into normal control group, model control group, Pudilan oral liquid group, and groups treated with differnet doses of Qingyan Lihou Formula. After 6 days of administration, the pharyngeal macroscopic status and histopathological changes were observed. The expression levels of PI3K, p-PI3K, AKT, and p-AKT in pharyngeal tissues were detected using the Wes system. Results: Network pharmacology analysis identified 62 active ingredients from Qingyan Lihou Formula and 208 corresponding targets associated with acute pharyngitis. The core targets included AKT1, PI3K, and IL6. KEGG enrichment analysis revealed that the PI3K-AKT signaling pathway was the key pathway. Molecular docking results showed that active ingredients such as quercetin and methylophiopogonone A exhibited good binding affinity with PI3K and AKT proteins, with binding energies all less than –6.0 kcal/mol. Animal experiments demonstrated that compared with the model control group, all Qingyan Lihou Formula treatment groups showed significantly improved pharyngeal secretions, congestion, and swelling, as well as reduced histopathological damage in pharyngeal tissues. The expression levels of PI3K, p-PI3K, AKT, and p-AKT in pharyngeal tissues were significantly decreased (P < 0.05, 0.01). Conclusion: Qingyan Lihou Formula may exert its therapeutic effects on acute pharyngitis by regulating the PI3K-AKT signaling pathway through active ingredients such as quercetin and methylophiopogonone A to suppress inflammatory responses. |
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