|
| 鱼腥草通过抑制鼻咽上皮细胞NF-κB信号通路抑制促炎细胞因子的产生 |
| Houttuynia cordata inhibits the production of pro-inflammatory cytokines by suppressing the NF - κ B signaling pathway in nasopharyngeal epithelial cells |
| 投稿时间：2025-06-30 修订日期：2025-06-30 |
| 中文关键词: 鱼腥草 鼻咽上皮细胞 NF-κB信号通路 促炎细胞因子 抗炎机制 |
| 英文关键词: Houttuynia cordata Nasopharyngeal epithelial cells NF - κ B signaling pathway Pro-inflammatory cytokines Anti-inflammatory mechanism |
| 基金项目: |
|
| 摘要点击次数: 20 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的：探讨鱼腥草乙醇提取物（HCT）对鼻咽上皮细胞炎症反应的抑制作用及其对核因子κB（NF-κB）信号通路的影响。方法：采用人鼻咽上皮细胞（NP69），以脂多糖（LPS）诱导炎症模型。细胞分别经不同浓度HCT（0.05、0.1、0.2 mg/mL）预处理1 h后给予LPS刺激。通过MTS法检测细胞活力；酶联免疫吸附试验（ELISA）检测细胞上清液中肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）水平；逆转录聚合酶链反应（RT-PCR）分析TNF-α、IL-6、IL-8 mRNA表达；蛋白质免疫印迹（Western blot）法检测IκBα磷酸化及NF-κB p65核转位；荧光素酶报告基因法测定NF-κB启动子活性。结果：与模型组比较，HCT能显著提高LPS诱导降低的细胞活力（P<0.05）；显著抑制LPS诱导的TNF-α、IL-6及IL-8分泌及其mRNA表达（P<0.05或0.01）；显著抑制IκBα磷酸化及NF-κB p65核转位（P<0.05或0.01）；显著下调NF-κB启动子活性（P<0.05或0.01）。结论：HCT可能通过抑制NF-κB信号通路活化，阻断IκBα降解及NF-κB核转位，从而抑制鼻咽上皮细胞促炎细胞因子产生，为中药治疗咽部炎症性疾病提供实验依据。 |
| 英文摘要: |
| Objective: To investigate the inhibitory effect of Houttuynia cordata ethanol extract (HCT) on the inflammatory response of nasopharyngeal epithelial cells and its impact on the nuclear factor kappa B (NF - κ B) signaling pathway. Method: Human nasopharyngeal epithelial cells (NP69) were used to induce an inflammatory model with lipopolysaccharide (LPS). Cells were pretreated with different concentrations of HCT (0.05, 0.1, 0.2 mg/mL) for 1 hour and then stimulated with LPS. Detect cell viability using MTS method; Enzyme linked immunosorbent assay (ELISA) was used to detect the levels of tumor necrosis factor - α (TNF - α), interleukin-6 (IL-6), and interleukin-8 (IL-8) in the cell supernatant; Reverse transcription polymerase chain reaction (RT-PCR) was used to analyze the mRNA expression of TNF - α, IL-6, and IL-8; Western blot was used to detect I κ B α phosphorylation and NF - κ B p65 nuclear translocation; Determination of NF - κ B promoter activity using luciferase reporter gene assay. Result: Compared with the model group, HCT significantly increased the cell viability induced by LPS (P<0.05); Significantly inhibited the secretion and mRNA expression of TNF - α, IL-6, and IL-8 induced by LPS (P<0.05 or 0.01); Significant inhibition of I κ B α phosphorylation and NF - κ B p65 nuclear translocation (P<0.05 or 0.01); Significantly downregulated NF - κ B promoter activity (P<0.05 or 0.01). Conclusion: HCT may inhibit the activation of the NF - κ B signaling pathway, block the degradation of I κ B α and NF - κ B nuclear translocation, thereby suppressing the production of pro-inflammatory cytokines in nasopharyngeal epithelial cells, providing experimental evidence for the treatment of pharyngeal inflammatory diseases with traditional Chinese medicine. |
|
View Fulltext
查看/发表评论 下载PDF阅读器 |
| 关闭 |
